tWO PHOTON facility INFM

National facility
for multi-photon excitation
fluorescence spectroscopy
on biomolecules

Sezione B

take a look at INFM microspheres! General Information Quick overview of the facility	*This is the home page of the INFM facility for non-linear spectroscopy and imaging in molecular biophysics.* The project has been funded by the INFM in 1999 and after a first period of construction and test of the basic configuration *it is now open for the INFM users. CALL FOR PROJECTS* This page gives references and information for possible applications and projects and calls for possible users. *Main applications* fluorescence imaging with 3D resolution. The high spatial localization of the two-photon excitation is exploited to obtain 3-D images of thick samples with no bleaching of the out of focus regions of the sample. The use of long wavelengths gives further advantages due to the low scattering of the excitation. lifetime decay Though the excitation is obtained through the absorption of two photons at a time, the emission properties are mostly unaffected with respect to the usual single photon excitation. This allows to perform studies of lifetime decays for molecules that cannot easily studied by single photon excitation. fluorescence polarization anisotropy decay Parallel to the lifetime studies we can measure the decay of the anisotropy of the fluorescence polarization. The use of two-photon excitation can help in studying dim fluorescent proteins since the static anisotropy for two-photon excitation is higher than the one usually obtained for single photon excitation. fluorescence correlation spectroscopy We exploit the high spatial resolution available with two-photon excitation to observe the fluorescence fluctuations. The minimum excitation volume available is 0.1 femtoLiters.
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Biophysics at the university of Milano-Bicocca	NOTE: Acrobat Reader is needed for some information in this site. If needed please download AcrobatReader